Journal Club, page 3

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Journal Club, page 3

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Enhanced Testing and Surveillance of Cryptosporidium

Cryptosporidium oocytes

Cryptosporidium/Giardia Journal Club

17 Nov 2025 Lora Benoit Ph.D

An optimized molecular-based workflow for detecting Cryptosporidium oocysts across complex agricultural matrices is described. The system integrates ddPCR with improved sample preparation and DNA recovery.
Food & Beverage Water

Multiplex PCR Detects Resistance Genes from Blood Cultures

Detection of Resistance Genes in Blood Cultures by Multiplex PCR

Antibiotic Resistance Blood Culture Journal Club

17 Nov 2025 Lora Benoit Ph.D

Marxreiter et al. (2025) benchmarked a rapid multiplex PCR assay against microdilution susceptibility testing, demonstrating how effectively molecular detection of resistance genes mirrors phenotypic resistance in Gram-negative pathogens.
Clinical & Veterinary

Comparison of Three Rapid Antimicrobial-Susceptibility-Testing Systems

Comparison of Rapid AST Systems like VITEK REVEAL

Antibiotic Resistance Antimicrobial Susceptibility Testing Journal Club

17 Nov 2025 Lora Benoit Ph.D

Squitieri et al. (2025) have conducted a head-to-head comparison of 3 rapid antimicrobial-susceptibility-testing (AST) systems applied directly to Gram-negative blood cultures in a clinical microbiology setting.
Clinical & Veterinary

Rapid Serotyping of Salmonella via MALDI-TOF and Machine Learning

Serotyping Salmonella Using MALDI-TOF and AI

Artifical Intelligence Journal Club MALDI-TOF

05 Nov 2025 Lora Benoit Ph.D

Study shows that combining MALDI-TOF MS with machine learning (XGBoost) can accurately identify Salmonella serotypes. This combined approach might soon provide diagnostic labs with a rapid and cost-effective approach to resolving Salmonella serotypes.
Food & Beverage Clinical & Veterinary

What Microbiology Labs Need to Know About Updates to Antimicrobial Resistance Testing in CLSI M100

Antimicrobial Resistance Testing in CLSI M100

Antibiotic Resistance Journal Club

15 Oct 2025 Lora Benoit Ph.D

In the new CLSI M100 edition, major updates were introduced - including restructured tables, new breakpoints, and a tiered approach to reporting.
Clinical & Veterinary

Everything You Need to Know About Equipment Validation for Sterility Testing

Sterility Testing of Cell and Gene Therapy Products

ATMP - Advanced Therapeutic Medicinal Products Cell and Gene Therapies Journal Club

14 Oct 2025 Lora Benoit Ph.D

As novel biologics and cell therapies advance, sterility testing is becoming increasingly complex and subject to cGMP scrutiny. Gebo et al. outline how clinical microbiology labs - traditionally CLIA/CAP-regulated - can align with FDA, USP, and ISO expectations.
Clinical & Veterinary Pharmaceutical

How Klebsiella pneumoniae Evolves and Persists in the Clinical Setting

How K pneumoniae Evolves and Persists in Clinical Setting

Journal Club Klebsiella pneumoniae carbapenemase (KPC)

13 Oct 2025 Lora Benoit Ph.D

Study on Klebsiella pneumoniae clone that evolved within patients over a 5-year hospital outbreak: virulence, capsule, iron scavenging, and biofilms shifted independently across hosts - highlighting how multidrug-resistant pathogens rapidly adapt and persist under clinical pressures.
Clinical & Veterinary

Tracing 20 Years of Salmonella Dublin Evolution: Human, Animal, Environmental Sources

Salmonella Spread Risk From Livestock

Journal Club Salmonella

04 Nov 2025 Lora Benoit Ph.D

In one of the most comprehensive studies of its kind, a genomic analysis of 2,150 Salmonella Dublin isolates collected in the U.S. over 20 years reveals strong genetic similarity among isolates from human, cattle, and environmental reservoirs.
Clinical & Veterinary

Legionella Detection in Water: Evaluating ISO 11731:2017 - Are All Methods the Same?

Best Way to Detect Legionella in Water

ISO 11731 - Legionella Legionella spp.

04 Nov 2025 Lora Benoit Ph.D

A multicenter study systematically compared six ISO 11731:2017 standardized culture techniques for detecting Legionella in 276 hospital hot-water samples. Detection rates varied significantly depending on the method used.
Water

A Defined Microbial Consortium Replicates Fine-flavor Chocolate Fermentation Traits

Precision Fermentation Produces Fine Chocolate Flavour

Journal Club

03 Nov 2025 Lora Benoit Ph.D

A rationally defined, 7-member microbial consortium assembled from isolated yeast and bacterial strains found during spontaneous cocoa fermentation was found to faithfully reproduce the chemical, sensory, and microbiological attributes of traditional fine-flavor chocolate.
Food & Beverage

Design of a Host-directed Adjuvant to Sensitize Intracellular Bacterial Persisters to Antibiotics

Intracellular Bacterial Persister Cells Contribute to Antibiotic Failure

Antibiotic Resistance Mycobacterium tuberculosis Salmonella Typhimurium

03 Nov 2025 Lora Benoit Ph.D

Lu et al., (2025) describes the discovery and characterization of a non-toxic small-molecule adjuvant, K1, which modulates the host cell in such a way as to resuscitate metabolically dormant intracellular bacteria making them become sensitive to antibiotics.
Clinical & Veterinary

Comparison of cgMLST Pipelines for Hospital Outbreak Detections

Comparison of cgMLST Pipelines for Hospital Outbreaks

Whole Genome Sequencing

03 Nov 2025 Lora Benoit Ph.D

Three commercial cgMLST pipelines were compared, using 255 hospital outbreak isolates, evaluating their concordance in identifying outbreak-related clusters and whether they consistently distinguish “clustered” vs “non-clustered” isolate pairs.
Clinical & Veterinary

Phage Cocktail Tailored to Treat Hospital Acquired Enterobacter cloacae Complex Infections

Multi-phage Formulations Attack Enterobacter cloacae complex

Coliphages Enterobacter Cloacae Complex (ECC)ESKAPE Pathogens

03 Nov 2025 Lora Benoit Ph.D

Study demonstrates that an institution-specific phage cocktail, built using local strain surveillance, can reduce time-to-effective therapy, curb transmission of resistant clones, and limit off-target ecological effects compared with broad, non-targeted antimicrobial agents.
Clinical & Veterinary

Blood Culture Contamination and Blood Culture Practices in US Hospitals: Time for Standardization?

Blood Culture Contamination

Blood Culture Journal Club Preanalytics

28 Oct 2025 Lora Benoit Ph.D

In one of the most comprehensive evaluations of its kind, Fabre et al. (2025) show striking variability in how hospitals define, measure, and act on blood culture contamination data, highlighting the need for national standardisation of definitions and reporting metrics.
Clinical & Veterinary

Campylobacter in the Domestic Kitchen: Breaking Bad Habits

How Domestic Kitchen Behaviour Can Influence Campylobacter Spread

Campylobacter Journal Club

08 Oct 2025 Lora Benoit Ph.D

Campylobacter is the leading cause of bacterial gastroenteritis in Europe. Improper handling of raw chicken - boosts Campylobacter cross-contamination risk in the kitchen; replacing cutting boards or knives was found to reduced pathogen transfer by 57–75%.
Food & Beverage

Seeds and Blossoms as Gateways: Internalization of Enteric Pathogens into Fresh Produce

Enteric Pathogens in Fresh Produce

E. coli E. coli inc VTEC/STEC Enterobacteriaceae

08 Oct 2025 Lora Benoit Ph.D

E. coli O157:H7 and Salmonella Typhimurium were inoculated onto lettuce and cucumber plants. The pathogens were frequently detected internally, indicating that these routes may allow entry into edible tissues and persist through harvest.
Food & Beverage

Invasive Fungal Diseases Are On The Rise

Climate Change Increasing Concern for Invasive Fungal Pathogens

Candida auris Journal Club

08 Oct 2025 Lora Benoit Ph.D

Konkel Neabore argues that rising temperatures, extreme weather events, and ecological disruption are accelerating the emergence, spread, and virulence of human fungal diseases. Mounting evidence links climate change to fungal adaptation and pathogenicity.
Clinical & Veterinary

Reducing Uncertainty in Bioburden Testing: A Statistical Framework for Pharma QA

Reducing Uncertainty in Bioburden Testing

Bioburden Journal Club

08 Oct 2025 Lora Benoit Ph.D

In biopharmaceutical manufacturing, variability in bioburden testing poses significant challenges for maintaining product quality. Here, a statistical framework designed to improve the interpretation of bioburden test outcomes - enhance regulatory compliance and increase consistency.
Pharmaceutical

Extended-Spectrum β-Lactamase–Producing ESKAPE-Es Detected in Fresh Produce Farms

Extended Spectrum ESKAPE Es

Antibiotic Resistance ESKAPE Pathogens Journal Club

08 Oct 2025 Lora Benoit Ph.D

A survey of smallholder farms in South Africa identified frequent contamination with extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales and Pseudomonas aeruginosa - informal farming systems may serve as reservoirs for multidrug-resistant bacteria.
Food & Beverage

Staphylococcus aureus Complex for the Clinical Microbiologist

Staphylococcus aureus Complex for the Clinical Microbiologist

Journal Club Staphylococcus aureus

01 Oct 2025 Lora Benoit Ph.D

A recent review examines the Staphylococcus aureus complex (SAC) - its clinical relevance, diagnostic challenges, and implications for epidemiology and infection control.
Clinical & Veterinary

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